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Image Search Results
Journal: International Journal of Biological Sciences
Article Title: ANXA1-mediated mTOR/FABP4 Inhibition Drives Antifibrotic Macrophage Reprogramming in Lupus Nephritis
doi: 10.7150/ijbs.118613
Figure Lengend Snippet: Ac2-26 administration treats established nephritis in MRL/ lpr mice. (A) Ac2-26 treatment protocol for MRL/ lpr mice. Ac2-26 (2 mg/kg) or PBS treatments were administered i.p. every other day from weeks 10 to 20. n = 6 per group. (B) Levels of anti-dsDNA antibodies in different groups at 10 weeks and 20 weeks (n = 6). (C) Serum creatinine levels in different groups at 10 weeks and 20 weeks (n = 6). (D) Urinary protein/creatinine ratios in different groups at 10 weeks and 20 weeks (n = 6). (E) Representative sections of renal tissue stained with H&E, Masson's trichrome, PAS, and PASM staining and semi-quantitative evaluation of glomerulonephritis at 20 weeks. Bars = 50 μm. (F) Representative photomicrographs and quantitative analysis of Sirius Red staining at 20 weeks. n = 5 per group. Bars = 50 μm. (G) Immunohistochemical staining and quantitative analysis of F4/80 at 20 weeks. n = 5 per group. Bars = 50 μm. (H) Immunohistochemical staining and quantitative analysis of osteopontin (encoded by Spp1 ) at 20 weeks. n = 5 per group. Bars = 50 μm. (I) Representative photomicrographs and quantitative analysis of Oil Red O staining at 20 weeks. n = 5 per group. Bars = 50 μm. (J) Immunohistochemical staining and quantitative analysis of Fabp4 at 20 weeks. n = 5 per group. Bars = 50 μm. Data analyses were performed by Student's t -test for two groups. * P < 0.05; ** P < 0.01; *** P < 0.001. i.p.: intraperitoneally; HE: hematoxylin-eosin; PAS: periodic acid-Schiff; PASM: periodic acid-silver methenamine.
Article Snippet: The
Techniques: Staining, Immunohistochemical staining